This result is in agreement with those of previous studies carried out in Colombia indeed, subgenotype F3 is the most frequent in the Andean region of the country, while genotype A is the most frequent HBV genotype in the western region (department of Chocó). Results: We identified genotypes A and F, subgenotype F3, in the samples. We also performed RFLP analysis in silico since we found differences between the patterns expected and those obtained We compared the patterns obtained with those in previous reports. We analyzed PCR products by RFLP with Alw I, Bsr I, Cfr I, Hpa II and Sty I, and we sequenced some. DNA extraction was followed by PCR amplification of a fragment of HBV ORF S. Materials and methods: We obtained 56 serum samples. However, due to the discrepancies of RFLP patterns it was also necessary to perform phylogenetic genotyping and in silico analysis of HBV sequences. Objective: The initial objective of the project was to identify the HBV genotypes by RFLP in serum samples obtained from patients and blood donors. One of the methodologies for determining the viral genotype is the restriction fragment length polymorphism (RFLP) technique, a simple and relatively inexpensive method, albeit with some limitations. Introduction: Ten viral genotypes (A-J) distributed in all continents have been described for hepatitis B virus (HBV). María Cristina Navas: conception and design of experiments, data analysis and drafting of the manuscriptĪlejandra Duque-Jaramillo: data analysis and drafting of the manuscriptĪll authors read and approved the final manuscript. Rendón: conception, design and performance of experiments, data analysis and drafting of the manuscriptįabián Cortés-Mancera: conception and design of experiments, data analysis Ospina 3, María Cristina Navas 1ġ Grupo de Gastrohepatología, Facultad de Medicina, Universidad de Antioquia, Medellín, ColombiaĢ Facultad de Ciencias Exactas, Instituto Tecnológico Metropolitano, Medellín, Colombiaģ Laboratorio Departamental de Salud Pública, Secretaría Seccional de Salud y Protección Social de Antioquia, Medellín, Colombia Rendón 1, Fabián Cortés-Mancera 1,2, Alejandra Duque-Jaramillo 1, Marta C. Analysis of hepatitis B virus genotypes by restriction fragment length polymorphism
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